General Cell Lines and Cell Cultures
ATCC Bacteroides fragilis
Bacteroides fragilis strain VPI 2553 [EN-2; NCTC 9343] is a type strain that was isolated from an appendix abscess. This whole-genome sequenced strain can be used in media testing, quality control, and pharmaceutical testing.
ATCC RAG; Renal Adenocarcinoma; Mouse (Mus musculus)
RAG is a cell line exhibiting amoeboid morphology that was isolated from the kidney of a mouse with renal adenocarcinoma disease. This cell line was deposited by RJ Klebe, FH Ruddle.
Eurofins Discoverx PathHunter U2OS TORC3 Nuclear Translocation Cell Line, 2 vials. (93-1120C3)
The PathHunter U2OS TORC3 Nuclear Translocation Cell Line is a stable, engineered cell line for use in studying drug candidates affecting the CRTC/CREB pathway. This cell line enables assessment of ligand (e.g. forskolin) based pathway activation via detection of resulting TORC3 (CRTC3) nuclear translocation. This product uses EFC technology. The included cell line overexpresses ProLabel-tagged TORC3 (CRTC3) and nuclear localized EA. Upon pathway activation, induced nuclear translocation of TORC3 leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/pathways.
Biochain Institute Inc Matched Pair - Total Protein - Human Primary Tumor and Normal Tissue (PP): Liver, 0.2 x2 mg /PK
BioChain's human total protein matched pair products include: Primary Pair (PP), Primary and Metastatic Pair (PM). PP consists of total protein isolated from primary tumor and its adjacent normal tissue; PM consists of total protein from primary tumor andcorresponding metastastic tumor. Total proteins in each pair are prepared from the same donor. This product line is designedfor identifying tumor-specific genes and tumor metastasis genes.
Eurofins Discoverx PathHunter GALR1 Total Internalization U2OS Cell Line, 2 vials. (93-0781C3)
The PathHunter GALR1 Total Internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of GALR1 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged GALR1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the GALR1 receptor to the early endosome leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter U2OS PDGFRa Functional Assay, 2 vials. (93-0823C3)
The PathHunter U2OS PDGFRa Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated PDGFRa dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged PDGFRa and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Eurofins Discoverx PathHunter U2OS Keap1-NRF2 Nuclear Translocation Cell Line, 2 vials. (93-0821C3)
The PathHunter U2OS Keap1-NRF2 Nuclear Translocation Cell Line is a stable, engineered cell line for use in studying drug candidates affecting the Keap1-NRF2 pathway. This cell line enables assessment of ligand (e.g. methyl CDDO) based pathway activation via detection of resulting NRF2 nuclear translocation. This product uses EFC technology. The included cell line overexpresses ProLabel-tagged NRF2 and nuclear localized EA alongside untagged Keap1. Upon pathway activation, induced nuclear translocation of NRF2 leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/pathways.
Eurofins Discoverx ChemiScreen κ Opioid Receptor Stable Cell Line, 2 vials. (HTS095C)
The ChemiScreen κ Opioid Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. DADLE, DPDPE) based activation of OPRK1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses OPRK1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of OPRK1, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
ATCC Pfizer Pneumococcal polysaccharide powder Type 26 (d6B) 10mg
Purified pneumococcal polysaccharide type 6B is manufactured by Pfizer, Inc., for small-quantity distribution by ATCC. The pneumococcal powder type 6B is a component of the current 13-valent vaccine against Streptococcus pneumoniae. Pneumococcal polysaccharide type 6B can be used to assay for type-specific antibodies, research immune responses, and study immunodeficiencies.
Eurofins Discoverx ChemiScreen β2 Adrenergic Receptor Membrane Preparation, 1 vial. (HTS073M)
The ChemiScreen β2 Adrenergic Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of ADRB2 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
ACROBiosystems HEK293/Human OX40 / TNFRSF4 / CD134 Stable Cell Line
Tumor necrosis factor receptor superfamily member 4 (TNFRSF4) is also known as ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor, CD antigen CD134, OX40. OX40 / TNFRSF4 contains four TNFR-Cys repeats. TNFRSF4 is receptor for TNFSF4 / OX40L / GP34 and can interacts with TRAF2, TRAF3 and TRAF5.
Montana Molecular Borealis Arrestin LPA1R Assay - 10mL
Measure arrestin signaling kinetics in real time with the live cell, fluorescent Borealis arrestin - LPA1 receptor assay. Add the unmodified LPA1 receptor and Borealis sensor to cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the arrestin sensor BacMam, LPA1R BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (AT1R BacMam and 5mM Angiotensin II in H2O). Borealis kits for several other receptors are also available, and larger-sized kits are available on request. Other Montana Molecular tools including the cADDis cAMP and DAG sensors can be used alongside this assay.
Eurofins Discoverx cAMP Hunter CHO-K1 MC3R Gs Cell Line, 2 vials. (95-0045C2)
The cAMP Hunter MC3R Gs cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. α-melanocyte stimulating hormone) based activation of MC3R GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type MC3R, a naturally Gs coupled GPCR. Upon activation of MC3R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
